Comparison of Ogawa and Löwenstein-Jensen media for Mycobacterium tuberculosis isolation in pulmonar tuberculosis. Regional Hospital of Trujillo, Peru

Abstract

The present investigation compare quantitative and qualitatively the culturing in Ogawa and Löwenstein Jensen media, for the isolation of M. tuberculosis in samples of saliva BK (+) in patients of Tuberculosis Program Regional Hospital of Trujillo, Peru. We tried 54 samples of saliva BK (+), being sowed by duplicate in the means of culture(culturing) in study and they were observed every three days for 2 months, the identification of M. tuberculosis was realized by means of the niacin test, according to the technology(skill) described by the National Institute of Health. One thought that 85.19 % of the samples was positive in both means; 39.13 % it(he,she) had growth eugénico in the way Ogawa and 28.26 % in the Löwenstein Jensen, having significant difference statistically. The difference of time of growth between both means was of 1.3 days favorably in the way Ogawa and the difference of the number of colonies between both means belonged to 7.43, not existing significant difference, in both. Therefore, one concludes that both means of culture(culturing) have the same efficiency in the isolation of M. tuberculosis.