Effect of lyophilized sap from Musa acuminata Colla on activation of peritoneal macrophages and antibody production in BALB/c mice infected with Escherichia coli O157:H7

Authors

  • Verónica Saravia Cueva Universidad Nacional de Trujillo, La Libertad, Perú
  • Manuela Luján Velásquez Universidad Nacional de Trujillo, La Libertad, Perú
  • Milciades Chávez Castillo Universidad Nacional de Trujillo, La Libertad, Perú
  • Lizzie Becerra Gutiérrez Universidad Nacional de Trujillo, La Libertad, Perú
  • Marianela Jiménez Coronado Universidad Nacional de Trujillo, La Libertad, Perú
  • Julio Cabeza Rodriguez Universidad César Vallejo, Lima, Perú

DOI:

https://doi.org/10.18050/revucv-scientia.v3i1.896

Keywords:

Musa acuminata, Escherichia coli, Mus musculus, Immune response

Abstract

This study attempted to determine the effect of lyophilized sap from Musa acuminata Colla on “in vitro” activation of peritoneal macrophages and antibody production in BALB/c mice infected with Escherichia coli O157:H7. Macrophage activation was determined by phagocytic index and microbicidal activity of murine peritoneal macrophages which were distributed in three groups: experimental A and experimental B, both had different concentrations of lyophilized sap (SLM), while control group had minimal essential medium. After that, we added E. coli O157:H7, so the concentration of SLM was 16,3 ug/mL in experimental A and 8,75 ug/mL in experimental B. Then we took samples in order to determine the phagocytic index and microbicidal activity. Antibody production was studied in BALB/c mice randomly allocated in three groups: experimental A, experimental B and control. Experimental A and B were orally administered with SLM at 20 mg/kg and 10 mg/kg respectively, control group was administered with sterile normal saline. They were infected with E. coli O157:H7 and then blood samples were collected in order to determine the antibody titer. Results showed that macrophages stimulated with SLM at 8,75 ug/mL had an increasing phagocytic index compared with the others. Besides microbicidal activity and antibody titer obtained in both experimental A and B were higher than control group. As a conclusion, lyophilized sap from Musa acuminata Colla increases phagocytic index, microbicidal activity and antibody production in BALB/c mice infected with Escherichia coli O157:H7.

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Published

2011-06-30

How to Cite

Saravia Cueva, V., Luján Velásquez, M., Chávez Castillo, M., Becerra Gutiérrez, L., Jiménez Coronado, M., & Cabeza Rodriguez, J. (2011). Effect of lyophilized sap from Musa acuminata Colla on activation of peritoneal macrophages and antibody production in BALB/c mice infected with Escherichia coli O157:H7. UCV-Scientia, 3(1), 42–48. https://doi.org/10.18050/revucv-scientia.v3i1.896

Issue

Vol. 3 No. 1 (2011): January - June

Section

Health

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Copyright (c) 2011 UCV - Scientia

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