Phytochemical screening of Plukenetia volubilis L. and its antioxidant effects of the 3+ Fe /ascorbate stimulated lipid peroxidation in hepatic of Rattus rattus var. albinus
DOI:
https://doi.org/10.18050/revucv-scientia.v2i1.808Keywords:
Plukenetia volubilis L., Antioxidant effect, Lipid peroxidation, Phytochemical screeningAbstract
This study was carried to determinated secondary metabolites of Plukenetia volubilis L. and analyze its 3+ antioxidant effects of the Fe /ascorbate stimulated lipid peroxidation in hepatic tissue of Rattus rattus var. albinus. Phytochemical screening was realized using Olga Lock technique, taking place the qualitative determination of the secondary metabolites by means of the coloration reactions and precipitation. Ethanol extract of Plukenetia volubilis L. was screened for in- vitro antioxidant activity using thiobartituric acid, measuring the produced malonaldialdehyde (MDA) to determinate lipid peroxidation in hepatic tissue homogenates in-vitro. Higher difference significant (p<0,001) was encountered with the minimum dosage (70 mg/mL) and maximum dosage (140 mg/mL) when was realized the matched dates “t” test, but there wasn't significant difference (p>0,05) when the minimum dosage and maximum dosage were analyzed. Ethanol extract showed presence of tannins, flavonoids, steroids, alkaloids and saponins on phytochemical screening. We demonstrated antioxidant activity of ethanol extract of Plukenetia volubilis L. in-vitro.
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