Phytochemical screening of Plukenetia volubilis L. and its antioxidant effects of the 3+ Fe /ascorbate stimulated lipid peroxidation in hepatic of Rattus rattus var. albinus

Authors

  • Ericson Felix Castillo Saavedra Universidad Católica los Ángeles de Chimbote, La Libertad, Perú
  • Segundo Felix Castillo Viera Universidad Nacional de Trujillo, La Libertad, Perú
  • Cecilia Elizabeth Reyes Alfaro Universidad Nacional de Trujillo, La Libertad, Perú

DOI:

https://doi.org/10.18050/revucv-scientia.v2i1.808

Keywords:

Plukenetia volubilis L., Antioxidant effect, Lipid peroxidation, Phytochemical screening

Abstract

This study was carried to determinated secondary metabolites of Plukenetia volubilis L. and analyze its 3+ antioxidant effects of the Fe /ascorbate stimulated lipid peroxidation in hepatic tissue of Rattus rattus var. albinus. Phytochemical screening was realized using Olga Lock technique, taking place the qualitative determination of the secondary metabolites by means of the coloration reactions and precipitation. Ethanol extract of Plukenetia volubilis L. was screened for in- vitro antioxidant activity using thiobartituric acid, measuring the produced malonaldialdehyde (MDA) to determinate lipid peroxidation in hepatic tissue homogenates in-vitro. Higher difference significant (p<0,001) was encountered with the minimum dosage (70 mg/mL) and maximum dosage (140 mg/mL) when was realized the matched dates “t” test, but there wasn't significant difference (p>0,05) when the minimum dosage and maximum dosage were analyzed. Ethanol extract showed presence of tannins, flavonoids, steroids, alkaloids and saponins on phytochemical screening. We demonstrated antioxidant activity of ethanol extract of Plukenetia volubilis L. in-vitro.

Published

2010-06-30

How to Cite

Castillo Saavedra, E. F., Castillo Viera, S. F., & Reyes Alfaro, C. E. (2010). Phytochemical screening of Plukenetia volubilis L. and its antioxidant effects of the 3+ Fe /ascorbate stimulated lipid peroxidation in hepatic of Rattus rattus var. albinus. UCV-Scientia, 2(1), 11–21. https://doi.org/10.18050/revucv-scientia.v2i1.808

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