Effect of Ethanolic Extract of Piper Aduncum Leaves, from Otuzco and Trujillo, on Human LDL Oxidation in Vitro

Abstract

The present research aims to study the effect of ethanolic extract from the leaves of Piper aduncum L., "matico" from different sources, on the oxidation of human low-density lipoproteins (LDL) in vitro. The leaves of the species were collected in January 2017 in the districts of Otuzco and Trujillo; the ethanolic extracts of Piper aduncum L. leaves were obtained by the Soxhlet method, and were evaporated to one third of their volume in a bain-marie, drying in an oven at 40°C for 24 hours; Solutions at a concentration of 0.2 mg /mL were prepared from the dry extract. LDL was isolated from blood samples of 81 persons using the Ultra Centrifugation Method; The inhibition degree of LDL oxidation was determined by the TBARS method, and three working groups were formed: a control group and two problem groups, according to the area of origin of the leaves. The concentration of malondialdehyde (MDA) in the control group is 0.0114 ± 0.00025 nmol/100 μL, in problem group 1 it is 0.0026 ± 0.0001 nmol/100 uL and in problem group 2 it is 0.0053± 0.00008 nmol/100 μL. It is concluded that the ethanolic extract of Piper aduncum L. leaves from Otuzco district inhibits human LDL oxidation by 77.04%, while the one from Trujillo district does so by 53.51%, in vitro.