Antifungal effect of Copaifera paupera oil-resin compared to fluconazole on Candida albicans: an in vitro study

Abstract

Objective: To evaluate the antifungal effectiveness of the oil – resin Copaifera paupera compared with fluconazol in Candida albicans. Material and Methods: It applied dilution techniques in solid milieu Tween 80 and diffusion in well by Kirby-Bauer’s technique in Agar Müeller-Hinton. It used strains ATCC 66027 of Candida albicans and the oil-resin of Copaifera paupera in concentration of 50 %, 25 % and 12,5 %. It established fluconazol (150 ug,) as positive control and Tween 80 as negative control. The hatching it made in 35 ± 2° C and the readings until 72 hours. Results: There weren’t halo’s formation of inhibition in Candida albicans strains in different dilutions of Copaifera paupera, Copaifera paupera + Tween 80 and Tween 80, in different from fluconazol with 150 ug it obtained one mean of 40 mm, its deviate with the mean of 1,56 mm and a range interquartile of 2 mm considering also the maximum and minimum value of 43 and 38 mm respectively. Conclusion: The Copaifera paupera is not effective as antifungal in Candida albicans, compared with fluconazol.