Antimicrobial effect of the ethanolic extract of Caesalpinia spinosa "tara" on Staphylococcus aureus ATCC 25923 compared to erythromycin

Abstract

Objective: To evaluate the antimicrobial effect of ethanol extract of Caesalpinia spinosa "tara" fruit (including the skin), on strains of Staphylococcus aureus ATCC 25923 compared to erythromycin in an in vitro study. Material and Methods: An experimental design was carried out with multiple post-test repetitions. The growth of microorganisms was observed in Petri-dishes. The ethanol extract was obtained by the method of filtration and maceration and for the antimicrobial susceptibility test, the Kirby-Bauer diffusion disk method was used. The ethanol extract of Caesalpinia spinosa "Tara" fruit (including the skin) was diluted at concentrations of 25 %, 50 %, 75 %, and 100 %. Erythromycin (500 mg), was set as a positive control and alcohol as the negative control. Incubation was carried out at 37° C and readings taken over a period of 24 hours. Results: According to the post ANOVA Tukey-test it can be observed that the major effect is provided by erythromycin (21,32 mm) followed by the dilution at 100 % (13,5 mm) and as the third-most-effective the dilution at 25 %, while 50 % and 75% were equal in terms of efficacy (ANOVA, p = 0,000). Conclusion: Treatment with erythromycin proved to be more effective and the ethanolic extract of Caesalpinia spinosa had lower effectiveness.